![]() This can be done very simply at the end of an experiment and it will allow you to archive and manage your data. The data recorded on Diva is saved as a file that can be read in Diva, however you will need to export the data out of Diva to generate FCS files that can be read on other software. The beads set the laser delays on the cytometer, test how well the lasers and detectors are working, and allows you to track the machine and alert your engineer if there are any problems. CS&T can be run as QC at whatever frequency you decide, some people run it occasionally, monthly, daily or before each experiment. The Cytometer Setting and Tracking (CS&T) beads are beads of different sizes and levels of fluorescence. Quality Controlįrom versions 6 onwards, the Diva software using CS&T to QC the cytometer, adjust some settings and to help you to set the optimal PMT voltages. Within each specimen you record your data in ‘tubes’. When running an experiment, you add specimens to your experiments meaning you can separate out your samples by cell type or different staining panels. This is really handy if you have a multi-user cytometer as each user can have a folder, which contains their data, or you can use a folder per month. It allows you to organise your work into folders and then separate experiments within the folders. Diva is a java-based software and is very user friendly. Therefore, the software has a lot of options for labelling your data files with information about your sample, such as adding keywords to the FCS files. Ease of useĭiva software can be used for research cytometry or for clinical samples. It is possible to upgrade the software but you may need to purchase new computer or video card. A majority of cytometers will have DIVA 6, which runs on XP, but all new BD cytometers now come with Windows 7 and DIVA 8. Over the years there have been different versions of the Diva software which all run on a PC but with different version of windows operating systems. You can use it for simple post-acquisition analysis such as gating, simple statistics and creating plots for your figures. However, Diva doesn’t have as many tools for post-acquisition analysis as programs such as FlowJo or FCSExpress. Diva software is used to acquire your cytometry data on LSRII, LSRFortessa, CantoII and Aria cell sorters.Īs well as acquiring your data using Diva software, you can also analyse your data after you have run your samples. Under USB HighSpeed Bus, click on USB DiskĦ.If you use a Becton Dickinson (BD) cytometer in your lab, the chances are you are acquiring your data using ‘Diva’ software. Click on the Storage tab, then click on Disk Utilityġ. Double-click the icon in the lower-right task bar that says “safely remove hardware”ģ. Select “Device Instance Path” from the drop-down listĤ. The dongle ID will appear as the last part of a line, everything after the slash mark (“\”)ġ. Navigate to Computer, then click on the dongle driveĢ. ![]() Right-click for Properties, then click “Hardware”Ĥ. Choose “Details”, then “Choose Device Instance Path”ĥ. The serial ID is everything after the last slash mark (“\”)ġ. Click “Desktop View”, then “file Explorer”ġ. Right-click on My Computer, then click “Properties”Ģ. Click the “Hardware” tab, then “Device Manager”ģ. Under Disk Drive, find the find the USB drive and double-click on itĥ. The Serial ID for the dongle will appear after the slash mark (“\”) Make sure that “Device Instance” is chosen.Ħ. If a FlowJo dongle breaks due to rough handling, such as twisting it in the computer, dropping a laptop, hitting it with a vacuum cleaner or foot, a new dongle will be sold to the researcher for a replacement fee plus shipping.If a FlowJo dongle is defective due to its manufacturing, FlowJo will replace it without cost, and the faulty dongle must be returned to FlowJo, LLC. If a FlowJo Dongle malfunctions, it is at the discretion of our dongle support team to replace or charge a replacement fee.The broken dongle must be returned to FlowJo, LLC. If the dongle is 3+ years old, there will most likely be a replacement fee, because the dongle probably has been used extensively.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |